E-mail: mariliamsilva@tecnico.ulisboa.pt
MSc in Biotechnology, Instituto Superior Técnico, University of Lisbon, Portugal, 2016
BSc in Biology, Instituto Superior de Agronomia, University of Lisbon, Portugal, 2014
In my work I aim to target a particular subset of breast cancer by using mesenchymal stem/stromal cells (MSCs) as cell vehicles for the local expression and delivery of a therapeutic protein, azurin. I will establish a stably transfected MSC cell line with azurin, with non-viral methods, namely the CRISPR/Cas9 system.
Silva, M., Monteiro, G.A., Fialho, A.M., Bernardes, N., da Silva, C.L. Conditioned Medium From Azurin-Expressing Human Mesenchymal Stromal Cells Demonstrates Antitumor Activity Against Breast and Lung Cancer Cell Lines. Front Cell Dev Biol 8:471 (2020).
Silva, M., Ulpiano, C., Bernardes, N., Monteiro, G.A., da Silva, C.L. Gene delivery as a tool to improve the therapeutic features of mesenchymal stromal cells: methods and applications in Gene Delivery: Methods and Applications, Zimmer, V. (ed.), Nova Science Publishers, ISBN 9781536162684 (2019).