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Research Associate

Education and Training

Post-Doctoral Fellow, Instituto Superior Técnico, University of Lisbon, Portugal, 2011-2016

PhD in Bioengineering, MIT Portugal Program, Instituto Superior Técnico, University of Lisbon, Portugal, 2011

MSc in Biotechnology (Biochemical Engineering), Instituto Superior Técnico, University of Lisbon, Portugal, 2007

Diploma in Biological Engineering, Instituto Superior Técnico, University of Lisbon, Portugal, 2005

Research activities

My research addresses the development of efficient, scalable and cost-effective production processes for the large-scale ex-vivo expansion of human adult stem cells (namely hematopoietic and mesenchymal stem cells) in controlled bioreactors.

Selected publications

Bucar, S., Branco, A.D.M., Mata, M.F., Milhano, J.C., Caramalho, I., Cabral, J.M.S., Fernandes-Platzgummer, A., da Silva, C.L. Influence of the mesenchymal stromal cell source on the hematopoietic supportive capacity of umbilical cord blood-derived CD34+-enriched cells. Stem Cell Res Ther 12:399 (2021).

de Almeida Fuzeta, M., Bernardes, N., Oliveira, F.D., Costa, A.C., Fernandes-Platzgummer, A., Farinha, J.P., Rodrigues, C.A.V., Jung, S., Tseng, R.-J., Milligan, W., Lee, B., Castanho, M.A.R.B., Gaspar, D., Cabral, J.M.S., da Silva, C.L. Scalable Production of Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Under Serum-/Xeno-Free Conditions in a Microcarrier-Based Bioreactor Culture System. Front Cell Dev Biol 8:553444 (2020).

Branco, A., Bucar, S., Moura-Sampaio, J., Lilaia, C., Cabral, J.M.S., Fernandes-Platzgummer, A., Lobato da Silva, C. Tailored Cytokine Optimization for ex vivo Culture Platforms Targeting the Expansion of Human Hematopoietic Stem/Progenitor Cells. Front Bioeng Biotechnol 8:573282 (2020).

Fernandes-Platzgummer, A., Rosa, S.S., Silva, R., Cunha, R., Fuzeta, M.A., Calado, C., Carvalho, C., Cabral, J.M.S., Azevedo, A., da Silva, C.L. Design and operation of a fully controlled platform for the production and purification of well-defined mesenchymal stromal cell (MSC)-derived extracellular vesicles. Cytotherapy 5:S48-S49 (2020).

Fernandes-Platzgummer, A., Andrade, P.Z., Cabral, J.M.S., da Silva, C.L. Scalable Manufacturing of Human Hematopoietic Stem/Progenitor Cells Exploiting a Co-culture Platform with Mesenchymal Stromal Cells. in Methods in Molecular Biology, Turksen, K. (ed.), Springer US, ISBN 9781071613269 (2020).

Mizukami, A., Neto, M.S.A., Moreira, F., Fernandes-Platzgummer, A., Huang, Y.-F., Milligan, W., Cabral, J.M.S., da Silva, C.L., Covas, D.T., Swiech, K. A fully-closed and automated hollow fiber bioreactor for clinical-grade manufacturing of human mesenchymal stem/stromal cells. Stem Cell Rev Rep 14(1):141-143 (2018).

de Soure, A.M., Fernandes-Platzgummer, A., Moreira, F., Lilaia, C., Liu, S.-H., Ku, C.-P., Huang, Y.-F., Milligan, W., Cabral, J.M.S., da Silva, C.L. Integrated culture platform based on a human platelet lysate supplement for the isolation and scalable manufacturing of umbilical cord matrix-derived mesenchymal stem/stromal cells. J Tissue Eng Regen Med 11(5):1630-1640 (2017).

Mizukami, A. Fernandes-Platzgummer, A., Carmelo, J.G., Swiech, K., Covas, D.T., Cabral, J.M.S., da Silva, C.L. Stirred tank bioreactor culture combined with serum-/xenogeneic-free culture medium enables an efficient expansion of umbilical cord-derived mesenchymal stem/stromal cells. Biotechnol J 11:1048-1059 (2016).

Fernandes-Platzgummer, A., Diogo, M.M., Lobato da Silva, C., Cabral, J.M.S. Maximizing Mouse Embryonic Stem Cell Production in a Stirred Tank Reactor by Controlling Dissolved Oxygen Concentration and Continuous Perfusion Operation. Biochem Eng J 82:81-90 (2014).

dos Santos, F., Campbell, A., Fernandes-Platzgummer, A., Andrade, P.Z., Gimble, J.M., Wen, Y., Boucher, S., Vemuri, M.C., da Silva, C.L., Cabral, J.M. A xenogeneic-free bioreactor system for the clinical-scale expansion of human mesenchymal stem/stromal cells. Biotechnol Bioeng 111:1116-1127 (2014).

Fernandes-Platzgummer, A., Diogo, M.M., Baptista, R.P., da Silva, C.L., Cabral, J.M.S. Scale-up of mouse embryonic stem cell expansion in stirred bioreactors. Biotechnol Prog 27(5):1421-1432 (2011).