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Research Associate

Education and Training

PhD in Biotechnology and Biosciences, Instituto Superior Técnico, University of Lisbon, Portugal, 2016

MSc in Biotechnology, Instituto Superior Técnico, University of Lisbon, Portugal, 2011

BSc in Biochemistry, Faculty of Sciences and Technology, New University of Lisbon, 2009

Research activities

My research at SCERG is focused on the expansion and directed differentiation of as three dimensional (3D) suspension aggregates. Human induced pluripotent stem cells (hiPSC) may be an ideal source for therapy because they can be derived from the patient to be treated, avoiding immune rejection, and they are also ideal for constructing disease models. However, to realize this enormous potential, it is necessary to develop robust and reproducible bioprocesses for human pluripotent stem cells (hPSCs) expansion and controlled differentiation at large scale. The main aim of my research is the development of a scalable and cost-effective culture platform for expansion of hPSCs and their controlled neural and cardiac differentiation under xeno-free chemically-defined conditions.

Selected publications

Miranda, C.C., Barata, T., Vaz, S.H., Ferreira, C., Quintas, A., Bekman, E.P. hiPSC-Based Model of Prenatal Exposure to Cannabinoids: Effect on Neuronal Differentiation. Front Mol Neurosci 13:119 (2020).

Miranda, C.C., Fernandes, T.G., Diogo, M.M., Cabral, J.M.S. Towards Multi-Organoid Systems for Drug Screening Applications. Bioengineering (Basel) 5(3). pii: E49 (2018).

Miranda, C.C., Fernandes, T.G., Pinto, S.N., Prieto, M., Diogo, M.M., Cabral, J.M.S. A scale out approach towards neural induction of human induced pluripotent stem cells for neurodevelopmental toxicity studies. Toxicol Lett 294:51-60 (2018).

Badenes, S.M., Fernandes, T.G., Miranda, C.C., Pusch-Klein, A., Haupt, S., Rodrigues, C.A.V., Diogo, M.M., Brüstle, O., Cabral, J.M.S. Long-term expansion of human induced pluripotent stem cells in a microcarrier-based dynamic system. J Chem Technol Biotechnol 92:492-503 (2017).

Miranda, C.C., Fernandes, T.G., Diogo, M.M., Cabral, J.M.S. Scaling Up a Chemically-Defined Aggregate-Based Suspension Culture System for Neural Commitment of Human Pluripotent Stem Cells. Biotechnol J 11(12):1628-1638 (2016).

Miranda, C.C., Fernandes, T.G., Pascoal, J.F., Haupt, S., Brüstle, O., Cabral, J.M.S., Diogo, M.M. Spatial and temporal control of cell aggregation efficiently directs human pluripotent stem cells towards neural commitment. Biotechnol J 10(10):1612-1624 (2015).