Post-Doctoral Fellow, Instituto de Medicina Molecular, University of Lisbon, Portugal, 1999-2017
PhD in Biomedical Sciences, University of Porto, Portugal, 1999
MSc in Embryology/Animal Physiology, Moscow State University, Russia, 1989
Invited Assistant Professor, Instituto Superior de Ciências Egas Moniz, Portugal (2010-2018)
My current research is focused on establishment of integrated platform bridging developmental biology and bioengineering for unravelling of disease mechanisms and new therapeutic approaches for neurodevelopmental disorders. My main focus is to develop 3D regionalized brain organoid models derived from patient induced pluripotent stem cells (iPSCs), including the use of CRISPR/Cas9 technology for generation of isogenic iPSC lines.
Complete list of publications can be seen at ORCID.
Silva, T.P., Sousa-Luís, R., Fernandes, T.G., Bekman, E.P., Rodrigues, C.A.V., Vaz, S.H., Moreira, L.M., Hashimura, Y., Jung, S., Lee, B., Carmo-Fonseca, M., Cabral, J.M.S. Transcriptome profiling of human pluripotent stem cell-derived cerebellar organoids reveals faster commitment under dynamic conditions. Biotechnol Bioeng 118(7):2781-2803 (2021).
Maranga, C., Vieira, A.A., Bekman, E.P., da Rocha, S.T. Induced pluripotent stem cells for modeling Angelman syndrome. in iPSCs for Modeling Central Nervous System Disorders, Birbrair, A. (ed.), Academic Press, ISBN 9780323857642 (2021).
Miranda, C.C., Barata, T., Vaz, S.H., Ferreira, C., Quintas, A., Bekman, E.P. hiPSC-Based Model of Prenatal Exposure to Cannabinoids: Effect on Neuronal Differentiation. Front Mol Neurosci 13:119 (2020).
Silva, T.P., Fernandes, T.G., Nogueira, D.E.S., Rodrigues, C.A.V., Bekman, E.P., Hashimura, Y., Jung, S., Lee, B., Carmo-Fonseca, M., Cabral, J.M.S. Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining. J Vis Exp 160: e61143 (2020).
Gomes, A.R., Fernandes, T.G., Vaz, S.H., Silva, T.P., Bekman, E.P., Xapelli, S., Duarte, S., Ghazvini, M., Gribnau, J., Muotri, A.R., Trujillo, C.A., Sebastião, A.M., Cabral, J.M.S., Diogo, M.M. Modeling Rett Syndrome With Human Patient-Specific Forebrain Organoids. Front Cell Dev Biol 8:610427 (2020).
Silva, T.P., Cotovio, J.P., Bekman, E., Carmo-Fonseca, M., Cabral, J.M.S., Fernandes, T.G. Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering. Stem Cells Int 2019:1-17 (2019).
Maranga, C., Fernandes, T.G., Bekman, E., da Rocha, S.T. Angelman syndrome: a journey through the brain. FEBS J 287(11):2154-2175 (2020).
Silva, T.P., Bekman, E., Fernandes, T.G., Vaz, S.H., Rodrigues, C.A.V., Diogo, M.M., Cabral J.M.S., Carmo-Fonseca, M. Maturation of human pluripotent stem cell-derived cerebellar neurons in the absence of co-culture. Front Bioeng Biotechnol 8:70 (2020).
Pereira, P.D., Serra-Caetano, A., Cabrita, M., Bekman, E., Braga, J., Rino, J., Santus, R., Filipe, P.L., Sousa, A.E., Ferreira, J.A. Quantification of cell cycle kinetics by EdU (5-ethynyl-2'-deoxyuridine)-coupled-fluorescence-intensity analysis. Oncotarget 8(25):40514-40532 (2017).
Ferreira, A.R., Silva, R.C., Abranches, E., Bekman, E., Henrique, D., Amaral, I.F., Pego, A.P. The endothelial cell-neural stem cell cross talk in the context of the development of spinal cord tissue engineering strategies. J Tissue Eng Regen Med 8:367-368 (2014).
Abranches, E., Bekman, E., Henrique, D. Generation and Characterization of a Novel Mouse Embryonic Stem Cell Line with a Dynamic Reporter of Nanog Expression. PLoS One 8(3):e59928 (2013).
Abranches, E., Silva, M., Pradier, L., Schulz, H., Hummel, O., Henrique, D., Bekman, E. Neural differentiation of embryonic stem cells in vitro: a road map to neurogenesis in the embryo. PLoS One 4(7):e6286 (2009).
Abranches, E., Bekman, E., Henrique, D., Cabral, J.M.S. Expansion of mouse embryonic stem cells on microcarriers. Biotechnol Bioeng 96(6):1211-1221 (2007).
Duarte, A., Hirashima, M., Benedito, R., Trindade, A., Diniz, P., Bekman, E., Costa, L., Henrique, D., Rossant, J. Dosage-sensitive requirement for mouse Dll4 in artery development. Genes Dev 18(20):2474-2478 (2004).
Bekman, E., Henrique, D. Embryonic expression of three mouse genes with homology to the Drosophila melanogaster prickle gene. Gene Expr Patterns 2(1-2):73-77 (2002).
