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Research Associate

Education and Training

PhD in Bioengineering, Instituto Superior Técnico, University of Lisbon, Portugal, 2019

Research Assistant, 3B's Research Group, University of Minho, Portugal, 2014-2015

MSc in Biomedical Engineering, Instituto Superior Técnico, University of Lisbon, Portugal, 2013

BSc in Biomedical Engineering, Instituto Superior Técnico, University of Lisbon, Portugal, 2011

Research activities

My research work is focused in the development of a new platform comprising 3D hierarchical scaffolds, mesenchymal stem/stromal cells (MSC) and bioreactor prototypes for the production of native-like articular cartilage. This project will employ the use of different biomanufacturing techniques such as extrusion, hydrogel synthesis and electrospinning to produce scaffolds able to mimic the complex stratified architecture of articular cartilage with different mechanical properties, extracellular matrix (ECM) composition and cell organization according with tissue location. Glycosaminoglycans (GAGs) known to have a role in the chondrogenic process will be studied and further incorporated within the scaffold structure. In addition, bioreactor prototypes able to provide different biophysical stimuli such as mechanical compressive loading and shear stress to promote chondrogenesis will be developed. We believe that our integrated strategy will play an important role in defining the optimal conditions for articular cartilage tissue engineering strategies.

Selected publications

Silva, J.C., Moura, C.S., Borrecho, G., Matos, A.P.A., Silva, C.L., Cabral, J.M.S., Bártolo, P.J., Linhardt, R.J., Ferreira, F.C. Extruded Bioreactor Perfusion Culture Supports the Chondrogenic Differentiation of Human Mesenchymal Stem/Stromal Cells in 3D Porous Poly (ε‐Caprolactone) Scaffolds. Biotechnol J doi: 10.1002/biot.201900078 [Epub ahead of print] (2019).

Silva, J.C., Udangawa, R.N., Chen, J., Mancinelli, C.D., Garrudo, F.F.F., Mikael, P.E., Cabral, J.M.S., Ferreira, F.C., Linhardt, R.J. Kartogenin-loaded coaxial PGS/PCL aligned nanofibers for cartilage tissue engineering. Mater Sci Eng C 107:110291 (2020).

Carvalho, M.S., Silva, J.C., Udangawa, R.N., Cabral, J.M.S., Ferreira, F.C., da Silva, C.L., Linhardt, R.J., Vashishth, D. Co-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineering. Mater Sci Eng C 99:479-490 (2019).

Silva, J.C., Carvalho, M.S., Udangawa, R.N., Moura, C.S., Cabral, J.M.S., da Silva, C.L., Ferreira, F.C., Vashishth, D., Linhardt, R.J. Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchymal stem/stromal cell osteogenesis towards a patient‐tailored bone tissue engineering approach. J Biomed Mater Res B doi: 10.1002/jbm.b.34554 [Epub ahead of print] (2020).

Silva, J.C., Moura, C.S., Borrecho, G., de Matos, A.P.A., Cabral, J.M.S., Linhardt, R.J., Ferreira, F.C. Effects of glycosaminoglycan supplementation in the chondrogenic differentiation of bone marrow- and synovial- derived mesenchymal stem/stromal cells on 3D-extruded poly (ε-caprolactone) scaffolds. Int J Poly Mat Poly Biomat doi: 10.1080/00914037.2019.1706511 [Epub ahead of print] (2020).

Silva, J.C., Carvalho, M.S., Han, X., Xia, K., Mikael, P.E., Cabral, J.M.S., Ferreira, F.C., Linhardt, R.J. Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices. Glycoconj J 36(2):141-154 (2019).

Aroso, I.M, Silva, J.C, Mano, F., Sá-Nogueira, I., Barreiros, S., Reis, R.L., Paiva, A., Duarte, A.R.C. Dissolution enhancement of active pharmaceutical ingredients by therapeutic deep eutectic systems. Eur J Pharm Biopharm 98:57-66 (2016).