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PhD Student

Education and Training

MSc in Biotechnology, Instituto Superior Técnico, University of Lisbon, Portugal, 2012

BSc in Chemical and Biological Engineering, Instituto Superior de Engenharia de Lisboa, Polytechnic Institute of Lisbon, Portugal, 2010

Research activities

The overall goal of my research is to develop scalable, integrated and innovative downstream processes for the purification of monoclonal antibodies (mAbs) based on clarification (cell harvest) by extraction using aqueous two-phase systems, and further purification by multimodal chromatographic systems operated under axial and/or radial modes. The thermodynamics of mAbs interaction with the different chromatography ligands and polymers are also of great interest in my research. I’m also focused in the optimization of the production of mAbs using mammalian cell technology.

Selected publications

Rosa, S.A., da Silva, C.L., Aires-Barros, M.R., Dias-Cabral, A.C., Azevedo, A.M. Thermodynamics of the adsorption of monoclonal antibodies in phenylboronate chromatography: Affinity versus multimodal interactions. J Chromatogr A 118-127 (2018).

Rosa, S.A., dos Santos, R., Aires-Barros, M.R., Azevedo, A.M. Phenylboronic acid chromatography provides a rapid, reproducible and easy scalable multimodal process for the capture of monoclonal antibodies. Sep Purif Technol 160:43-50 (2016).

dos Santos, R., Rosa, S.A., Aires-Barros, M.R., Tover, A., Azevedo, A.M. Phenylboronic acid as a multi-modal ligand for the capture of monoclonal antibodies: development and optimization of a washing step. J Chromatogr A 1355:115-124 (2014).